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. 2019 Jun 27;7:150. doi: 10.3389/fbioe.2019.00150

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Copyright © 2019 Joachim, Maguire, Schäfer, Gerlach and Czermak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence-based assay to test the activity of IMPI against thermolysin. A replicate assay lacking IMPI served as a negative control, and phosphoramidon was included as a positive control. Error bars represent the standard deviation of triplicates. (A) ELP-IMPI(I38V) produced using LB complex medium, induction at 37°C with 1.0 mM IPTG. (B) ELP-IMPI(I38V) produced using M9i minimal medium, induction at 40°C with 1.1 mM IPTG.