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. 2019 Jun 27;7:150. doi: 10.3389/fbioe.2019.00150

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Copyright © 2019 Joachim, Maguire, Schäfer, Gerlach and Czermak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of induction to maximize the recombinant protein concentration (c_P) using Equation (12), shown as 3D plots. (A) Concentration of inducer against temperature after induction for 6 h. (B) IPTG concentration against the duration of induction at a constant temperature of 40°C. Confirmation runs (■) under the optimal conditions predicted by the model were performed using biological triplicates.