Figure 10.

Adoptive transfer of CX₃CR1⁺ DCs attenuates JE progression. (A) Attenuated infiltration of Ly-6C^hi monocytes and Ly-6G^hi granulocytes in the CNS of CX₃CR1^−/− mice injected with CX₃CR1⁺CD11c⁺ DCs. (B) Accumulated number of infiltrated Ly-6C^hi monocytes and Ly-6G^hi granulocytes in the CNS of CX₃CR1^−/− mice injected with CX₃CR1⁺CD11c⁺ DCs. CNS-infiltrated leukocytes were obtained from the brains of CX₃CR1^−/− recipients of CX₃CR1⁺CD11c⁺ DCs with vigorous cardiac perfusion and collagenase digestion at 3 dpi. CX₃CR1^+/+ wild-type mice and CX₃CR1^−/− mice that received no cells were used as positive and negative controls, respectively. Values in the dot-plots represent the average percentage of each population after gating on CD45⁺ and subsequent CD11b⁺ cells. (C) Expression of inflammatory cytokines and chemokines in the CNS of CX₃CR1^−/− mice injected with CX₃CR1⁺CD11c⁺ DCs. Expression of cytokines and chemokines was determined by real-time qRT-PCR using total RNA extracted from brain tissue at indicated dpi. Data show the average ± SEM of levels derived from at least three independent experiments (n = 4–5). ^*p < 0.05; ^**p < 0.01; and ^***p < 0.001 comparing CX₃CR1^−/− mice and CX₃CR1^−/− recipients of CX₃CR1⁺CD11c⁺ DC at indicated dpi.