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. 2019 Jun 27;13:673. doi: 10.3389/fnins.2019.00673

FIGURE 4.

FIGURE 4

The PERK-dependent pathway of the UPR is up-regulated in PARK20 fibroblasts. (A,B) Western blot analysis of the relative expression of markers of the PERK-dependent pathway of UPR. Starved HDF (WT) and PARK20 cells were either untreated (C) or treated for 2 h with 1 μM GSK2606414 (GSK) or 500 nM Thapsigargin (TG) alone (GSK and TG respectively) or in combination (TG + GSK). For TG + GSK samples, a pre-treatment of 30 min with GSK2606414 was performed prior to the addition of TG. α-Tubulin (TUB) was used as loading control. (B) Densitometry analyses of three independent experiments are shown. Histogram shows the relative fold induction of the expression of the indicated proteins in the treated samples compared to the control samples. Results are expressed as mean values ± SD; ∗p ≤ 0.05, ∗∗p ≤ 0.01, Student’s t-test.