Table 2.
Antibody | Manufacturer | Cat no | Dilution (1% BSA in TBST) |
Secondary (in TBST) |
---|---|---|---|---|
Anti-Actin | Sigma | A3853 | 1:5000 | Mouse |
Anti-β-Catenin | Cell Signaling | 2698 | 1:1000 | Mouse |
Anti-phospho-β-Catenin | Cell Signaling | 9561 | 1:1000 | Rabbit |
Anti-c-Myc | Abcam | AB32 | 1:1000 | Mouse |
Anti-phospho-CRMP2 (Ser509/514) | In house | 1:1000 | Sheep | |
Anti-phospho-CRMP2 (Ser522) | In house | 1:1000 | Sheep | |
Anti-total CRMP2 | Cell Signaling | 9393 | 1:1000 | Rabbit |
Anti-phospho-CRMP4 (Ser509) | In house | 1:1000 | Sheep | |
Anti-total Glycogen synthase | Cell Signaling | 3886 | 1:1000 | Rabbit |
Anti-phospho-Glycogen synthase | Cell Signaling | 3891 | 1:1000 | Rabbit |
Anti-GSK3 α/β(for IB) | Cell Signaling | 5676 | 1:1000 | Rabbit |
Anti-phospho GSK3 α/β (S21/9) | Cell Signaling | 8566 | 1:1000 | Rabbit |
Anti-GSK3α (for IP) | Abcam | Ab40870 | 1 μg/0.1mg of lysate | Rabbit |
Anti-GSK3β (for IP) | In House | 1 μg/0.1mg of lysate | Sheep | |
Anti-total ERK (p44/42 MapK) | Cell Signaling | 9102 | 1:1000 | Rabbit |
Anti-phospho-ERK (Thr202/Tyr204) | Cell Signaling | 9101 | 1:1000 | Rabbit |
Anti-total PKB | Cell Signaling | 2920 | 1:1000 | Mouse |
Anti-phospho-PKB (Thr308) | Cell Signaling | 9275 | 1:1000 | Rabbit |
Anti-phospho-PKB (Ser473) | Cell Signaling | 9271 | 1:1000 | Rabbit |
Anti-S6 | Cell Signaling | 2217 | 1:1000 | Rabbit |
Anti-phospho-S6 (Ser240/244) | Cell Signaling | 2215 | 1:1000 | Rabbit |
Anti-Tau5 | Millipore | MAB361 | 1:1000 | Mouse |
Anti-phospho-Tau (Thr231) | Millipore | AB9668 | 1:1000 | Rabbit |
Anti-phospho-Tau (Ser396) | Cell Signaling | 9632 | 1:1000 | Mouse |
VASP | In house | 1:1000 | Sheep | |
HRP conjugated anti-RABBIT IgG | Thermo Fisher | S1440 | 1:2500 | N/A |
Anti-RABBIT IgG (H&L) IRDye800®Conjugated | Rockland | 613-132-122 | 1:10000 | N/A |
Anti-RABBIT IgG (H&L) IRDye800®Conjugated | Licor | 925-32211 | 1:5000 | N/A |
Anti-SHEEP IgG (H&L) IRDye800®Conjugated | Rockland | 613-431-002 | 1:10000 | N/A |
Anti-MOUSE IgG (H+L) Alexa Fluor 680 | Life Technologies | A21057 | 1:5000 | N/A |