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. 2019 Jun 28;12:163. doi: 10.3389/fnmol.2019.00163

Table 2.

Antibodies used in this work.

Antibody Manufacturer Cat no Dilution
(1% BSA in TBST)
Secondary
(in TBST)
Anti-Actin Sigma A3853 1:5000 Mouse
Anti-β-Catenin Cell Signaling 2698 1:1000 Mouse
Anti-phospho-β-Catenin Cell Signaling 9561 1:1000 Rabbit
Anti-c-Myc Abcam AB32 1:1000 Mouse
Anti-phospho-CRMP2 (Ser509/514) In house 1:1000 Sheep
Anti-phospho-CRMP2 (Ser522) In house 1:1000 Sheep
Anti-total CRMP2 Cell Signaling 9393 1:1000 Rabbit
Anti-phospho-CRMP4 (Ser509) In house 1:1000 Sheep
Anti-total Glycogen synthase Cell Signaling 3886 1:1000 Rabbit
Anti-phospho-Glycogen synthase Cell Signaling 3891 1:1000 Rabbit
Anti-GSK3 α/β(for IB) Cell Signaling 5676 1:1000 Rabbit
Anti-phospho GSK3 α/β (S21/9) Cell Signaling 8566 1:1000 Rabbit
Anti-GSK3α (for IP) Abcam Ab40870 1 μg/0.1mg of lysate Rabbit
Anti-GSK3β (for IP) In House 1 μg/0.1mg of lysate Sheep
Anti-total ERK (p44/42 MapK) Cell Signaling 9102 1:1000 Rabbit
Anti-phospho-ERK (Thr202/Tyr204) Cell Signaling 9101 1:1000 Rabbit
Anti-total PKB Cell Signaling 2920 1:1000 Mouse
Anti-phospho-PKB (Thr308) Cell Signaling 9275 1:1000 Rabbit
Anti-phospho-PKB (Ser473) Cell Signaling 9271 1:1000 Rabbit
Anti-S6 Cell Signaling 2217 1:1000 Rabbit
Anti-phospho-S6 (Ser240/244) Cell Signaling 2215 1:1000 Rabbit
Anti-Tau5 Millipore MAB361 1:1000 Mouse
Anti-phospho-Tau (Thr231) Millipore AB9668 1:1000 Rabbit
Anti-phospho-Tau (Ser396) Cell Signaling 9632 1:1000 Mouse
VASP In house 1:1000 Sheep
HRP conjugated anti-RABBIT IgG Thermo Fisher S1440 1:2500 N/A
Anti-RABBIT IgG (H&L) IRDye800®Conjugated Rockland 613-132-122 1:10000 N/A
Anti-RABBIT IgG (H&L) IRDye800®Conjugated Licor 925-32211 1:5000 N/A
Anti-SHEEP IgG (H&L) IRDye800®Conjugated Rockland 613-431-002 1:10000 N/A
Anti-MOUSE IgG (H+L) Alexa Fluor 680 Life Technologies A21057 1:5000 N/A