Table 2.
Data of the relative expression fold changes for each gene at the different time intervals are indicated for each biological replicate. Each value was calculated from the mean of triplicate Cq values used in the Pfaffl equation. The mean fold change is indicated in the last three columns.
Gene | Biological Replicate 1 |
Biological Replicate 2 |
Mean Relative Expression Fold1 Change |
||||||
---|---|---|---|---|---|---|---|---|---|
Relative Expression at 4h | Relative Expression at 8h | Relative Expression at 24h | Relative Expression at 4h | Relative Expression at 8h | Relative Expression at 24h | 4h | 8h | 24h | |
ompA | 1.022 | 0.967 | 2.676 | 1.230 | 1.278 | 5.521 | 1.126 | 1.123 | 4.099 |
ompF | 0.277 | 0.465 | 0.550 | 0.558 | 0.287 | 0.681 | 0.418 | 0.376 | 0.616 |
Fold change values <1 indicate a decrease in expression, = 1 no change in expression and >1 indicates increase in expression levels relative to the control cells lacking hlyF.