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. 2019 Jul 3;9:9607. doi: 10.1038/s41598-019-46119-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myocardial cross sections of IR and IR + MCP-treated rats and quantification of Gal-3 expression. (A) Representative microphotographs of myocardial sections were stained with Masson trichrome and imaged at 4x and 40x power fields. (B) Ischemic areas were selected and quantified using computed-based image analysis software. (C,D) Cardiomyocyte cross sectional area was measured in myocardial sections. (E) Quantification of GDF-15 protein expression. (F) Quantification of Gal-3 protein levels in sham, IR and IR + MCP rats. (G) Representative microphotographs imaged at 40x of the ischemic zone stained for Gal-3. (H) Quantification of Gal-3 downstream proteins RCN-3 and fumarase. Histogram bars represent the mean ± SEM of each group of animals (n ≥ 12 per group) in arbitrary units or as a percentage of staining normalized to HPRT, 18 s and β-actin or stain-free gel for cDNA and protein respectively. *p < 0.01 vs. Sham, $, p < 0.01 vs.IR.