Table 1.
Effect of the inhibition of Gal-3 on cardiac parameters in IR rats.
| Sham | IR | IR + MCP | |
|---|---|---|---|
| Weights | n = 10 | n = 12 | n = 10 |
| Body (g) | 323 ± 5 | 297 ± 7* | 294 ± 8* |
| Heart (mg) | 1036 ± 22 | 974 ± 27 | 992 ± 31 |
| LV (mg) | 719 ± 17 | 691 ± 21 | 698 ± 22 |
| HW/BW (mg/g) | 3.21 ± 0.06 | 3.29 ± 0.09 | 3.38 ± 0.06 |
| LVW/BW (mg/g) | 2.23 ± 0.05 | 2.33 ± 0.06 | 2.38 ± 0.05 |
| Function (MRI) | n = 10 | n = 9 | n = 9 |
| HR (bpm) | 403 ± 11 | 349 ± 12* | 343 ± 18* |
| LVESV (μl) | 102 ± 5 | 190 ± 15*** | 224 ± 22*** |
| LVEDV (μl) | 352 ± 14 | 433 ± 22* | 466 ± 32** |
| EF (%) | 71 ± 1 | 57 ± 2*** | 52 ± 2*** |
| SV (µl) | 250 ± 10 | 244 ± 12 | 242 ± 17 |
| CO (ml/min) | 100 ± 3 | 84 ± 4* | 83 ± 6* |
| Perfusion (MRI) | n = 10 | n = 10 | n = 10 |
| LV perf (ml/mg/min) | 6.09 ± 0.19 | 3.64 ± 0.15*** | 4.27 ± 0.14***,† |
| Septum perf (ml/mg/min) | 6.35 ± 0.22 | 3.75 ± 0.17*** | 4.60 ± 0.17***,†† |
| Hemodynamics | n = 8 | n = 8 | n = 7 |
| HR (bpm) | 417 ± 14 | 394 ± 14 | 379 ± 13 |
| SBP (mmHg) | 132 ± 5 | 124 ± 5 | 121 ± 3 |
| DBP (mmHg) | 94 ± 4 | 92 ± 5 | 90 ± 3 |
| LVESP | 125.7 ± 3.2 | 121.9 ± 5.9 | 111.9 ± 5.3 |
| LVEDP | 2.2 ± 0.5 | 4.0 ± 0.9 | 2.9 ± 0.7 |
| dP/dt max | 9784 ± 294 | 8283 ± 452 | 7968 ± 572* |
| dP/dt min | 8504 ± 392 | 6889 ± 362* | 6591 ± 321** |
| LVESPVR | 24.38 ± 0.98 | 17.83 ± 0.90*** | 17.19 ± 0.91*** |
| LVEDPVR | 0.82 ± 0.09 | 2.32 ± 0.30*** | 1.51 ± 0.12† |
| Tau e | 5.34 ± 0.20 | 7.63 ± 0.32*** | 6.71 ± 0.43* |
| Tau 1/2 | 4.19 ± 0.18 | 5.29 ± 0.38* | 4.44 ± 0.22 |
| Serum parameters | n = 10 | n = 10 | n = 10 |
| BNP (AU) | 0.16 ± 0.014 | 0.26 ± 0.024* | 0.19 ± 0.024† |
| IL-1 β (AU) | 127 ± 14 | 184 ± 12* | 139 ± 11† |
| CRP (AU) | 2667 ± 410 | 4231 ± 425* | 2436 ± 235† |
| Hemodynamics | n = 8 | n = 8 | n = 7 |
| HR (bpm) | 417 ± 14 | 394 ± 14 | 379 ± 13 |
| SBP (mmHg) | 132 ± 5 | 124 ± 5 | 121 ± 3 |
| DBP (mmHg) | 94 ± 4 | 92 ± 5 | 90 ± 3 |
| LVESP | 125.7 ± 3.2 | 121.9 ± 5.9 | 111.9 ± 5.3 |
| LVEDP | 2.2 ± 0.5 | 4.0 ± 0.9 | 2.9 ± 0.7 |
| dP/dt max | 9784 ± 294 | 8283 ± 452 | 7968 ± 572* |
| dP/dt min | 8504 ± 392 | 6889 ± 362* | 6591 ± 321** |
| LVESPVR | 24.38 ± 0.98 | 17.83 ± 0.90*** | 17.19 ± 0.91*** |
| LVEDPVR | 0.82 ± 0.09 | 2.32 ± 0.30*** | 1.51 ± 0.12† |
| Tau e | 5.34 ± 0.20 | 7.63 ± 0.32*** | 6.71 ± 0.43* |
| Tau 1/2 | 4.19 ± 0.18 | 5.29 ± 0.38* | 4.44 ± 0.22 |
| Serum parameters | n = 10 | n = 10 | n = 10 |
| BNP (AU) | 0.16 ± 0.014 | 0.26 ± 0.024* | 0.19 ± 0.024† |
| IL-1 β (AU) | 127 ± 14 | 184 ± 12* | 139 ± 11† |
| CRP (AU) | 2667 ± 410 | 4231 ± 425* | 2436 ± 235† |
IR: ischemia reperfusion; MCP: modified citrus pectin; LV: Left ventricle; LVW: LV weight; BW: Body weight; HW: heart weight; HR: heart rate; LVESV: LV end-systolic volume; LVEDV: LV end-diastolic volume; EF: ejection fraction; SV: stroke volume; CO: cardiac output; SBP: systolic blood pressure; DBP: diastolic blood pressure; LVESP: LV end-systolic pressure; LVEDP: LV end-diastolic pressure; LVESPVR: LV end-systolic pressure-volume relation; LVEDPVR: LV end-diastolic pressure-volume relation; BNP: bran natriuretic peptide; IL-1 β: interleukin-1 beta; CRP: C-reactive protein. Data values represent mean ± SEM number of rats per group is indicated in each column. *p < 0.05, **p < 0.01 *** p < 0.001 vs. control group; †p < 0.05 vs. IR group. Anova plus Tukey for weight and functional measurements and plus Bonferroni for protein quantification.