Figure 3.

Restoration of FVIII Expression in C-iECs

(A) Immunofluorescence staining of iECs expressing markers for CD31 (red) and CD144 (green). DAPI was used to visualize the nucleus. (B) RT-PCR analysis of F8 expression in iECs. Primers based on exon 14 (E14) and primers based on exons 23 and 26 (E23–E26) were used to detect F8 expression. GAPDH was used as a loading control. (C) FVIII antigen levels in ECs derived from iPSCs were measured by ELISA. Bars represent the mean ± SEM (n = 3, independent cultures). (D) FVIII activity in the supernatants of differentiated ECs. The FVIII activity of the HA-iECs was lower than the detection limit of the instrument. No significant difference in FVIII activity was observed between C-iECs (2-6-iECs and 2-46-iECs) and N-iECs. Bars represent the mean ± SEM (n = 3, independent cultures). ns, not significant, compared with N-iECs.