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. 2019 Jul 3;9:9596. doi: 10.1038/s41598-019-46116-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Corneal opacity induced by alkali burn is suppressed in NLRP3 KO mice cornea. Representative photographs are shown. Compared with WT mice (a, opacification grade 4; intense stromal opacity, only outline of pupil is visible), the opacification is suppressed in NLRP3 KO mice (b, opacification grade 2; minimal to moderate opacity including corneal stroma). Using opacification grade, it is shown that corneal opacity in NLRP3 KO mice (b) is significantly diminished compared with WT mice (a) (N = 10 each, WT mice; 3.5 ± 0.5, NLRP3 KO mice; 2.0 ± 1.2, *P < 0.05, MannWhitney U-test) on day 7 after alkali burn injury (c).