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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Structure. 2019 May 30;27(7):1057–1070.e4. doi: 10.1016/j.str.2019.04.014

Figure 1. NatA and Naa50 form stable complexes in yeast and human.

Figure 1.

(A) Gel filtration elution profiles of Naa50 with NatA in S. pombe (top),human (middle), and cross-species between yeast S. pombe and S. cerevisiae (bottom), using a Superdex S200 column, with either 200 mM or 1M NaCl in sizing buffer. Coomassie-stained SDS–PAGE of peak fractions are shown to the right of the chromatograms.

(B) Fluorescence polarization assays with NatA titrated into fluorescein-5-maleimide labeled Naa50 in both S. pombe (left) and human (right) systems. The data is fit to calculate a dissociation constant between NatA and Naa50.

(C) Differential scanning fluorimetry assays of NatA alone or with Naa50 in both yeast and human systems. Recorded melting temperature transitions are indicated.