Figure 8.

Fluorescence Microscopy of ftNPs (Nile Red) and Intracellular Distribution in hCAECs in TNF-α-Stimulated Cells after 4 hours and 1 and 2 Days of Incubation

Z-stack-assembled (25–40 slices) fluorescence images of hCAECs cultured with unloaded or loaded NPs, and stained to highlight cell nucleus (Hoechst; nucleic acids; blue) and endoplasmic reticulum (protein disulfide isomerase; enzyme; green) in monolayer cultured under static conditions. Scale bars represent 20 μm. Images are representative of three independent experiments. Color settings were optimized for loaded NPs at day 1 of incubation, and then used for all images, apart from the nuclear staining.