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. 2019 Jun 12;17:223–234. doi: 10.1016/j.omtn.2019.06.001

Figure 3.

Figure 3

miRNA-26a-5p Accelerates Osteoblast Activity and Matrix Mineralization

(A) miRNA-26a-5p was upregulated in agomiR-26a-5p-treated MC3T3-E1 cells after transfection for 48 h with the Lipofectamine control or 200 μM agomiR-26a-5p, agomiR-NC, antagomiR-26a-5p, or antagomiR-NC. (B–E) qPCR was used to assess the expression of osteoblast differentiation genes, including Col1a1 (B), ALP (C), OCN (D), and Runx2 (E) in MC3T3-E1 cells treated for 48 h with Lipofectamine control, agomiR-26a-5p, agomiR-NC, antagomiR-26a-5p, or antagomiR-NC. (F) Western blot analysis of ALP, Col1a1, OCN, and Runx2 protein levels in MC3T3-E1 cells treated for 48 h with Lipofectamine control, agomiR-26a-5p, agomiR-NC, antagomiR-26a-5p, or antagomiR-NC. (G) ALP staining in MC3T3-E1 transfected for 48 h with Lipofectamine control, agomiR-26a-5p, agomiR-NC, antagomiR-26a-5p, or antagomiR-NC. Scale bar, 10 mm. (H) Alizarin red-mediated calcium staining in MC3T3-E1 cells after transfection for 21 days with Lipofectamine control, agomiR-26a-5p, agomiR-NC, antagomiR-26a-5p, or antagomiR-NC. Scale bar, 10 mm. Data are means ± SD of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.