KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Senl | Chen et al., 2014 (Dave Brow) | N/A |
| Anti-Nrd1 | Steinmetz and Brow, 1998 (Dave Brow) | N/A |
| Bacterial and Virus Strains | ||
| E. coli BL21 (DE3) Star | Novagen | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Sodium citrate | Fisher Scientific | S279-3 |
| Lithium chloride | Sigma-Aldrich | 203637-50G |
| PEG 6000 | Sigma-Aldrich | 81260-1KG |
| ScSen1-NIM1: EQEDDYKLPMEYIT | GL Biochem | N/A |
| ScSen1-NIM2-FAM: DDEDDYTPSISD-[FAM] | GL Biochem | N/A |
| ScSen1-NIM2-Y2058F-FAM: DDEDDFTPSISD-[FAM] | GL Biochem | N/A |
| ScSen1-NIM2-P2060A: DDEDDYTASISD | GL Biochem | N/A |
| ScSen1-NIM2: DDEDDYTPSISD | GL Biochem | N/A |
| ScSen1-NIM2-P2060V: DDEDDYTVSISD | GL Biochem | N/A |
| ScSen1-NIM2-D2056N: DDENDYTPSISD | GL Biochem | N/A |
| ScSen1-NIM3: EAEDPYDLNPHPQ | GL Biochem | N/A |
| Pol II CTD: SPT(pS)PSYSPT(pS)PS | GL Biochem | N/A |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Nrd1 CID | Vasiljeva et al., 2008 | PDB: 3CLJ |
| Nrd1 CID in complex with Sen1 NIM1 | This paper | 6O3W |
| Nrd1 CID in complex with Sen1 NIM2 | This paper | 6O3X |
| Nrd1 CID in complex with Sen1 NIM3 | This paper | 6O3Y |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Yeast strains | ||
| YSB3181 (SEN1 shuffling strain) | Mischo et al, 2018 | N/A |
| nrd2-1/YF1517 | Steinmetz and Brow, 1996 (Dave Brow) | N/A |
| 46a/YF1313 | Steinmetz and Brow, 1996 (Dave Brow) | N/A |
| Oligonucleotides | ||
| Forward primer used to clone Nrd1-CID construct in pET28a C AGC GGC CTG GTG CCG CGC GGC AGC CAT ATG GAT TTT CAA AAT TTT GTA GCT ACC TTG G |
Eton Bioscience | N/A |
| Reverse primer used to clone Nrd1-CID construct in pET28a GTG GTG GTG GTG GTG CTC GAG TGC GGC CGC CTA AGC AAA GCA TTT TGA CCT GAT GGC |
Eton Bioscience | N/A |
| Forward primer used to clone Sen1 constructs in pET28a-SUMO GGC CTG GTG CCG CGC GGC AGC CAT ATG xxx xxx indicates coding-sequence-complementary region of variable length |
Eton Bioscience | N/A |
| Reverse primer used to clone Sen1 constructs in pET28a-SUMO GTG GTG GTG CTC GAG TGC GGC CGC CTA xxx xxx indicates coding-sequence-complementary region of variable length |
Eton Bioscience | N/A |
| Forward primer used to clone Nrd1-CID construct in pCDF GT TTA ACT TTA ATA AGG AGA TAT ACC ATG GGC GAT TTT CAA AAT TTT GTA GCT ACC TTG G |
Eton Bioscience | N/A |
| Reverse primer used to clone Nrd1-CID construct in pCDF C TGT TCG ACT TAA GCA TTA TGC GGC CGC CTA AGC AAA GCA TTT TGA CCT GAT GGC |
Eton Bioscience | N/A |
| Recombinant DNA | ||
| pET28a-Nrd1-CID | This study | N/A |
| pET28a-SUMO-Sen1 | This study | N/A |
| pCDF-Nrd1-CID | This study | N/A |
| Software and Algorithms | ||
| XDS | Kabsch, 2010 | http://xds.mpimf-heidelberg.mpg.de/ |
| PHENIX | McCoy, 2007; Adams, 2002 | https://www.phenix-online.org/ |
| Coot | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PyMOL | Schrodinger LLC | https://pymol.org |
| GraphPad Prism | GraphPad Software | www.graphpad.com |
| Other | ||