KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
None
Bacterial and Virus Strains
DH5 alpha High efficiency competent cells	Invitrogen	Cat No. 18258012
BL21DE3	New England Biolabs	Cat No. C 25271
Biological Samples
None
Chemicals, Peptides, and Recombinant Proteins
n-Undecyl-β-D-Maltopyranoside, Anagrade	Anatrace	Cat No. U300LA
Façade®-EM 3α-hydroxy-7α,12α-di-((O-β-D-maltosyl)-2-hydroxyethoxy)-cholane	Avanti Polar Lipids	Cat No. 850552
Lipid A, diphosphoryl from E. coli F583	Sigma Aldrich	Cat No. L5399
ATP Adenosine 5’-triphosphate (ATP) disodium salt hydrate	Sigma Aldrich	Cat No. FLAAS
β-Nicotinamide adenine dinucleotide hydrate	Sigma Aldrich	Cat No. N 7004
L-Lactic dehydrogenase from rabbit muscle	Sigma Aldrich	Cat No. L 2500
Sodium orthovanadate	Sigma Aldrich	Cat. No. S6508
CPM (7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin)	Thermo Fisher	Cat No. D 346
Critical Commercial Assays
None
Deposited Data
MsbA 2.8 Å crystal structure	This paper	6BL6
MsbA 4.46 Å crystal structure	This paper	6O30
Experimental Models: Cell Lines
None
Experimental Models: Organisms/Strains
None
Oligonucleotides
None
Recombinant DNA
pET19b	Novagen	Cat No. 71146
Software and Algorithms
GraphPad Prism 5	GraphPad	N/A
Other
PDB files used in this study	https://www.rcsb.org/	3B60, 3B5X, 3B5W, 5TV4, 6BPL