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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Cell Rep. 2019 Jun 4;27(10):2962–2977.e5. doi: 10.1016/j.celrep.2019.05.013

Figure 4. Otx1, Vegt, and Foxh1 Form an Assembly in the Chromatin.

Figure 4

(A) Venn diagram of Otx1, Vegt, and Foxh1 biologically reproducible peak overlaps.

(B) Genome browser view of Otx1, Vegt, and Foxh1 binding, highlighting 4 regions of peak overlaps near the bpm7.2, pou5f3.3, fgf20, and nodal6 genes.

(C) Sequential ChIP-qPCR using anti-Otx1 followed by anti-FLAG, anti-Vegt, or anti-Foxh1 antibody in regions of peak overlap highlighted in (B). ins and id3 promoters were used as negative controls. The error bars represent variation from 3 technical replicates.

(D) Foxh1 morpholino KD followed by Otx1 or Vegt ChIP-qPCR performed on stage 8 embryos. eef1a1 and odc1 promoters were used as negative control. Shown is a single representative biological replicate. The error bars represent the variation from 3 technical replicates.