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. 2019 Jul 3;12:103. doi: 10.1186/s12920-019-0553-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Visual verification of variants with Integrative Genomic Viewer (IGV) and sequencing chromatogram with secondary confirmation test results. Variants with low fractions in IGV reflect NGS results from analyzing peripheral blood. The corresponding sequencing chromatograms are the results of MEMO-PCR of peripheral blood for P1 and P2, conventional PCR of polyp tissue for P3 and P5, and conventional PCR of peripheral blood for P6 and P7