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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell. 2019 Mar 28;177(2):299–314.e16. doi: 10.1016/j.cell.2019.02.013

Figure 6. Low Mitochondrial Permeability Is Required for Autophagy-Dependent Lifespan Extension.

Figure 6.

(A and B) Basal and maximal (FCCP) oxygen consumption (A) and ATP level (B) in sgk-1 mutants with empty vector (ev) or bec-1 RNAi. (n = 12, two-way ANOVA; n = 7, student’s t-test).

(C) Cytochrome C western blot in sgk-1 mutants following RNAi of bec-1, lgg-1 or unc-51 (n = 4, one-way ANOVA).

(D) bec-1 RNAi does not extend lifespan in mev-1(kn1);sgk-1 double mutants.

(E) Larval knockdown of drp-1 extends lifespan in sgk-1 mutant worms (log rank test).

(F-I) sgk-1 loss of function or overexpression of vdac-1 eliminate autophagy-dependent lifespan extension in both eat-2(ad465) mutants (F and H) and glp-1(e2141) mutants (G and I) (log rank test).

(J) RNAi of frh-1 and nuo-6 extends lifespan in wild type worms but has no effect on vdac-1 transgenic worms (log rank test).

See also Figure S6. For tabular survival data and biological replicates see also Table S1. *p < 0.05, **p < 0.01, ****p < 0.0001. All bars indicate means and SEM.