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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell. 2019 Mar 28;177(2):299–314.e16. doi: 10.1016/j.cell.2019.02.013

Figure 7. Mice lacking Sgk Are More Sensitive to Liver Ischemia/Reperfusion (I/R) Injury.

Figure 7.

(A) Hepatic warm I/R injury model.

(B and C) Serum ALT (B) and AST (C) levels in 12-week-old Sgk1Lko versus control Sgk1flox/flox mice after hepatic I/R injury (n = 10 per group, student’s t-test).

(D) Active, cleaved caspase 3 and LC3A/B II protein levels mice after hepatic I/R injury (n = 5 for control and n = 6 for Sgk1Lko, student’s t-test).

(E) mRNA levels of inflammatory genes IL-1β and IL-6 by QPCR (n = 11 per group, student’s t-test).

(F and G) Serum ALT (F) and AST (G) levels of 24-week-old SgkTKO mice versus Sgk1flox/flox control after hepatic I/R injury with or without cyclosporine A (CsA) pretreatment (n =8 for control and SgkTKO, and n = 7 for SgkTKO+CsA, one-way ANOVA).

(H) Active, cleaved caspase 3 and LC3A/B protein levels in liver after hepatic I/R injury with or without CsA pretreatment (n =7 for control and SgkTKO+CsA, and n = 8 for SgkTKO, two-way ANOVA).

(I) Model of the effect of autophagy on lifespan with facilitated opening of the mPTP (mPTP open) versus normal mitochondrial permeability (mPTP closed) conditions.

*p < 0.05, **p < 0.01. All bars indicate means and SEM.