Fig. 2.

Effect of serum free media in the development of day 7 blastocysts. a Representative bright field (left) and immunostaining (right) images of bovine IVF embryos cultured in SOF 0.8% BSA and N2B27. b Number of cells per embryo cultured under two conditions. Data are presented as mean ± S.E. The number of embryos analysed was 5 and 7 produced in SOF and N2B27, respectively, from three independent replicates. Different superscripts show significant differences between groups determined by Fisher’s exact test (p value< 0.05). Scale bar: 10 μm