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. 2019 Jul 4;19:13. doi: 10.1186/s12861-019-0193-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of low dose of MEK inhibitor on NANOG and SOX17 expression in day 8 IVF bovine embryos. a Immunostaining detection of NANOG and SOX17 in representative day 8 bovine IVF blastocysts. Each image is a single optical section. b Scatter plots showing NANOG and SOX17 positive cells from control and treatments. Data were analysed by Kruskal Wallis and are presented as Median ± IQR. Different superscripts indicate significant difference between groups (p < 0.05). N: number of embryos counted after immunofluorescence detection, N = 7; 4; 6 and 9 for DMSO, 0.4 μM PD032, 1 and 2 μM PD032 group, respectively. Scale bar: 10 μm