Table 2.
Effect of high dose of PD0325901 in bovine embryos
| Treatments | n | N | Total Cells | ICM | TE | ICM:TE |
|---|---|---|---|---|---|---|
| DMSO | 5 | 9 | 235 ± 17a | 86 ± 7a | 148 ± 14c | 0,60 ± 0,11 a |
| PD032 (2.5 μM) | 3 | 8 | 238 ± 18a | 28 ± 3c | 210 ± 20a | 0,13 ± 0,03 b |
| PD032 (5 μM) | 3 | 12 | 252 ± 18a | 37 ± 3b | 214 ± 20a | 0,17 ± 0,03 b |
| PD032 (10 μM) | 3 | 11 | 202 ± 14b | 36 ± 3b | 82 ± 15b | 0,16 ± 0,04 b |
Number of inner cell mass (ICM), trophoblast (TE) and total cells in embryos cultured under different conditions. “n” is the number of independently IVF runs and “N” is the number of embryos analyzed. Values are mean ± S.E. estimated by a GLMM analysis with Poisson distribution and log as link function (for Total Cells, ICM and TE), and with Binomial distribution with logit as link function for (ICM:TE ratios). Different letters denote significant differences, p < 0.05