Table 3.

Effect of t2iGöLIF in bovine embryos

Treatments	n	N	Total cells	ICM	TE	ICM:TE	NANOG:ICM
DMSO	4	15	261 ± 15a	53 ± 7c	210 ± 20a	0,25 ± 0,05	0,45 ± 0,02b
t2iGö	3	10	282 ± 17a	75 ± 10b	198 ± 19a	0,39 ± 0,08	0,53 ± 0,04a
h-LIF	3	10	281 ± 17a	91 ± 12a	180 ± 18b	0,50 ± 0,08	0,42 ± 0,01b
t2iGö h-LIF	3	9	209 ± 13b	55 ± 8c	142 ± 14c	0,38 ± 0,08	0,59 ± 0,02a

Number of inner cell mass (ICM), trophectoderm (TE) and total cells in embryos cultured under different conditions. “n” is the number of independently IVF runs and “N” is the number of embryos analyzed. Values are mean ± S.E. estimated by a GLMM analysis with Poisson distribution and log as link function (for Total Cells, ICM and TE), and with Binomial distribution with logit as link function for (ICM:TE and NANOG:ICM ratios). Different letters denote significant differences, p < 0.05