Fig. 5. AGR of serum samples from 2D-IR spectroscopy. (a and b) AGR obtained using the 2D-IR diagonal method. (c and d) AGR obtained using the pump slice method. (e and f) AGR values obtained using the 2D-IR linear combination method. The spectral basis of the method is shown in (b), (d) and (f) respectively. (g) AGR obtained from averaging the results in (a), (c) and (e). In panels (a), (c), (e) and (g) the solid black line indicates the actual AGR of the samples. Error bars show 2σ variation. Dashed lines show linear fits to the experimental AGR values.