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. 2019 Jun 28;10:1434. doi: 10.3389/fimmu.2019.01434

Figure 2.

Figure 2

Effect of OECs on DC maturation and cytokine expression. (A) Expression of CD86, CD80, CD40 and HLA-DR in monocyte-derived DCs cultured alone (DC Ctrl) or in co-culture with OECs during 48 h (DC:H413; DC:TR146; DC: primary OECs). Black bars represent cells incubated with MV130 and gray bars represent cells in media. DCs are gated based on CD11c staining. (B) Cytokine secretion by DCs cultured with OECs. Figure shows the levels of TNFα, IL-12, IL-6, IL-8, IL-10 and TGFβ (pg/ml) determined by ELISA in supernatants of DCs cultured alone (DC Ctrl) or in co-culture with OECs during 48 h (DC:H413; DC:TR146; DC: primary OECs). Black bars represent cells incubated with MV130 and gray bars represent cells with media. Statistically significant differences (p < 0.05) between the different DC conditions and DC Ctrl were noted as “#” for media or as “*” for MV130. Data were obtained from a total of four independent experiments and we plotted mean values with error bars corresponding to SEM.