Table 3. Mutational stability prediction of substituted amino acids at B-cell epitopes of E.
| Mutations | mCSM | Effect | SDM | Effect | DUET | Effect | I-Mutant2.0 | Effect |
|---|---|---|---|---|---|---|---|---|
| T309A | - 0.464 Kcal/mol | Destabilizing | 0.12 Kcal/mol | Stabilizing | -0.22 Kcal/mol | Destabilizing | -0.15 Kcal/mol | Decreased
stability |
| T335A | - 0.596 Kcal/mol | Destabilizing | 0.12 Kcal/mol | Stabilizing | -0.337 Kcal/mol | Destabilizing | 0.33 Kcal/mol | Increased
stability |
| R335T | - 0.304 Kcal/mol | Destabilizing | -0.38 Kcal/mol | Destabilizing | -0.173 Kcal/mol | Destabilizing | NA | NA |
| V391I | - 0.375 Kcal/mol | Destabilizing | 0.08 | Stabilizing | -0.022 | Destabilizing | -0.25 Kcal/mol | Decreased
stability |
| D393E | - 0.38 Kcal/mol | Destabilizing | 0.06 Kcal/mol | Stabilizing | -0.149 Kcal/mol | Destabilizing | NA | NA |