Fig. 1.

Exposures to iAs and MAs³⁺ decreased glycogen content and increased free glucose concentration in primary murine hepatocytes. Hepatocytes were exposed to iAs³⁺ (a, c) or MAs³⁺ (b, d) for 4 h with or without insulin treatment (100 nM) for the last 2 h of the exposure. Glycogen (a, b) and free glucose (c, d) were measured in cell lysates. MTT assay was used to assess cell viability in hepatocytes exposed to iAs³⁺ (e) or MAs³⁺ (f) for 4 h with insulin treatment (100 nM) for the last 2 h of the exposure. Values are expressed as mean and SD for three independent experiments. *Statistically significant differences (p < 0.05) between control hepatocytes and control hepatocytes treated with insulin, and #between insulin-treated control hepatocytes and insulin-treated hepatocytes exposed to arsenicals; p values for marginally significant differences are also shown