Table 2.
Activities of StDHODH and PiDHODH with alternative electron acceptors.
| Co-substrate | Activity % StDHODH | Activity % PiDHODH |
|---|---|---|
| Decylubiquinone (Qd) | 100 ± 7 | 100 ± 2 |
| Ubiquinone (Q0) | 96 ± 9 | 56 ± 1 |
| Ubiquinone (Q1) | 124 ± 11 | 111 ± 1 |
| Ubiquinone (Q2) | 123 ± 4 | Not done |
| Ubiquinone (Q6) | 102 ± 3 | 144 ± 2 |
| Ubiquinone (Q9) | 111 ± 2 | 66 ± 5 |
| Ubiquinone (Q10) | 19 ± 4 | 30 ± 1 |
| 2,5-Dimethyl-p-benzoquinone | 67 ± 6 | 48 ± 1 |
| 5,8-Dihydroxy-1,4-naphthoquinone | 113 ± 7 | 17 ± 3 |
| 1,4-Naphthoquinone | 78 ± 5 | 50 ± 1 |
| 2-Hydroxy-1,4-naphthoquinone (lawsone) | 14 ± 7 | 5 ± 1 |
| 5-Hydroxy-1,4-naphthoquinone (juglone) | 5 ± 1 | 15 ± 1 |
| 5-Hydroxy-2-methyl-1,4-naphthoquinone (plumbagin) | 46 ± 6 | 6 ± 1 |
| 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) | 17 ± 7 | 23 ± 1 |
| Ferricyanide | 3.5 ± 0.4 | 1.6 ± 1.0 |
Activities were measured with the standard DCIP assay using 0.1 mM of the electron acceptor indicated, and 1 mM dihydroorotate. The activity with Qd was set as 100%.