Table 3.
Activities of StDHODH and PiDHODH in the presence of potential inhibitors.
| Inhibitor | Activity % StDHODH | Activity % PiDHODH |
|---|---|---|
| 5′-Fluorotic acid (5′-FOA) | 55 ± 4 | 83 ± 3 |
| Alloxan | 74 ± 20 | 112 ± 4 |
| A77-1726 | 97 ± 6 | 14 ± 3 |
| Dichloroallyl lawsone (DCL) | 39 ± 6 | 30 ± 2 |
| Toltrazuril (1 mM) | 0 | 67 ± 4 |
| Brequinar | 49 ± 1 | 98 ± 3 |
| Redoxal | 39 ± 3 | 31 ± 8 |
| DSM190 | 38 ± 5 | 36 ± 10 |
| MD 108 | 90 ± 4 | 23 ± 4 |
| MD 129 | 95 ± 8 | 103 ± 2 |
| MD 209 | 0 | 2.0 ± 0.5 |
| MD 241 | 87 ± 3 | 1.0 ± 0.7 |
| NSC 61890 | 36 ± 2 | 93 ± 2 |
| NSC 71097 | 70 ± 10 | 129 ± 4 |
| NSC 277965 | 130 ± 8 | 60 ± 2 |
| Atovaquone | 75 ± 6 | 127 ± 39 |
| Ametoctradin | 102 ± 5 | 41 ± 1 |
Activities were measured with the standard DCIP assay with saturating concentrations of Qd (0.1 mM) and dihydroorotate (1 mM), in the presence of 0.5 mM inhibitor, unless otherwise indicated. The activity without inhibitor was set as 100%. Compounds that increase activity above 100% apparently acted as electron acceptors instead of inhibitors.