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. 2019 Jun 28;6:97. doi: 10.3389/fnut.2019.00097

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This work is authored by RP and EG on behalf of the U.S. Government and, as regards RP and EG and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Apparent gluten concentration (μg/mL) profiles of gluten -reduced barley beers (RA, RB, RC, RD, RE, RF), obtained by the multiplex-competitive ELISA utilizing gluten specific antibodies from the AgraQuant Gluten G12 (a), GlutenTox ELISA Competitive G12 (b), RIDASCREEN Gliadin (c), RIDASCREEN Gliadin Competitive (d), Veratox for Gliadin, cat # 8480 (e), Neogen Veratox for Gliadin R5 (f), Microbiologique Gluten (g), Morinaga Institute of Biological Sciences, Inc. (MIoBS) Gliadin (h), and AllerTek Gluten (25 (i) and 10 μg/mL (j) coating antigen concentration) ELISA kits (56). (B) Western blot binding signal of gluten-reduced barley beers (RA, RB, RC, RD, RE, RF), using the detector antibodies of Veratox for Gliadin, cat # 8,480 and AllerTek Gluten ELISA kits. Lane information for western blot: Lane 1- Molecular weight marker, Lane 2- Empty, Lane 3- 2.5 μg/mL intact gluten standard, Lane 4- Empty, Lane 5- beer RA, Lane 6- Empty, Lane 7- beer RB, Lane 8- Empty, Lane 9- beer RC, Lane 10- Empty, Lane 11- beer RD, Lane 12- Empty, Lane 13- beer RE, Lane 14- Empty, Lane 14- beer RF (49).