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. 2019 Jun 28;9:235. doi: 10.3389/fcimb.2019.00235

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Copyright © 2019 Ehmen and Lüder.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of in vitro infection of monocyte-enriched PBMCs with T. gondii on subset distribution and expression of CD14 and CD16 of monocytes from blood donors with or without chronic toxoplasmosis. (A) Monocyte-enriched PBMCs were isolated from blood samples and were either directly FACS-analyzed (0 h) or cultivated in vitro for 48 h and parasite-infected or not during the final 24 h as indicated and then FACS-analyzed. (B–D) Percentages of CD14⁺CD16⁻ (B), CD14⁺CD16⁺ (C) and CD14^dimCD16⁺ (D) subsets among monocytes from T. gondii seropositive (gray bars) or seronegative (open bars) individuals. (E,F) Expression levels of CD14 (E) and CD16 (F) on monocytes from T. gondii seropositive or seronegative blood donors. Data represent means ± S.E.M. from 5 T. gondii seropositive and from 13 out of 16 seronegative blood donors which had been randomly selected for in vitro infection assays; outlyers were excluded. Individual data points are also indicated. Significant differences between groups were identified by ANOVA (^***p < 0.001; ^**p < 0.01; ^*p < 0.05).