Skip to main content
. 2019 Jun 28;9:235. doi: 10.3389/fcimb.2019.00235

Figure 5.

Figure 5

Regulation of mRNA levels of IL-12b, IL-10, and TNFa in monocyte-enriched PBMCs from blood donors with or without chronic toxoplasmosis after in vitro infection with T. gondii. Monocyte-enriched PBMCs were isolated from blood samples and blood donors were serologically classified as chronically T. gondii-infected or non-infected using blood plasma. Cells were either directly extracted for RNA isolation or they were cultivated for 48 h and parasite-infected (dark gray bars) or not (light gray bars) during the final 24 h. After RNA isolation, mRNA was reverse transcribed and cDNA amplified by quantitative real-time PCR using primer pairs specific to IL-12b (A), IL-10 (B), TNFa (C), and ß-actin. Cytokine mRNA levels were normalized to ß-actin mRNA, and the regulation was calculated between cells directly isolated after blood donation and those after in vitro infection with T. gondii (dark gray bars) or after control cultivation (light gray bars). Bars represent means ± S.E.M. from 5 T. gondii seropositive and seronegative blood donors each; outlyers were excluded. Individual data points are also indicated. Significant differences between groups were identified by ANOVA (***p < 0.001; **p < 0.01; *p < 0.05); bars labeled with different letters differ significantly.