Table 1.
Two-way ANOVA assessment of effect of Time, Diet, and Interaction in microglia.
| Genes | Two-way ANOVA analysis | ||
|---|---|---|---|
| p-value | |||
| Interaction | Time | Diet | |
| Circadian | |||
| Bmal1 | 0.0356 | <0.0001 | 0.3308 |
| Clock | 0.0006 | 0.4015 | 0.9599 |
| Cry1 | <0.0001 | 0.0002 | 0.1484 |
| Cry2 | <0.0001 | 0.1616 | 0.7026 |
| Per1 | 0.0011 | 0.0437 | 0.9673 |
| Per2 | <0.0001 | 0.0035 | 0.9989 |
| Reverba | 0.0023 | <0.0001 | 0.5351 |
| Dbp | <0.0001 | <0.0001 | 0.0573 |
| Inflammatory | |||
| Tnfa | 0.1547 | 0.0042 | 0.1705 |
| Il1b | 0.0085 | 0.2610 | 0.4658 |
| Myd88 | 0.0378 | 0.0022 | 0.0140 |
| Ikbkb | <0.0001 | 0.0165 | 0.7572 |
| Cd68 | 0.0685 | 0.0226 | 0.0014 |
| Sirt1 | 0.0004 | 0.2071 | 0.4654 |
| Metabolic | |||
| Gls | 0.0138 | 0.4456 | 0.0888 |
| Gdh | 0.0095 | 0.8306 | 0.8648 |
| Gpx1 | 0.0009 | 0.9390 | 0.8424 |
| Hk2 | 0.0023 | 0.0274 | 0.0024 |
| Glut5 | 0.0058 | 0.0935 | 0.0025 |
| Cd36 | 0.0031 | 0.0004 | 0.1116 |
| Lpl | 0.0554 | 0.0064 | 0.0412 |
| Ppard | 0.0034 | 0.6143 | 0.7868 |
| Fas | 0.0030 | 0.5214 | 0.6840 |
| Mitochondrial | |||
| Cox4 | <0.0001 | 0.1598 | 0.8468 |
| Atp5b | 0.0020 | 0.0257 | 0.0194 |
| Pdk4 | 0.2512 | <0.0001 | 0.7817 |
| Fis1 | 0.0001 | 0.3438 | 0.0934 |
| Drp1 | <0.0001 | 0.2189 | 0.6340 |
| Mfn2 | 0.0001 | 0.4675 | 0.1899 |
| Opa1 | 0.0056 | 0.9284 | 0.6975 |
Diet, Time, and Interaction effects were evaluated in microglia for circadian, inflammatory, metabolic, and mitochondrial genes. Statistical significance was determined using Two-way ANOVA effect for Interaction, Diet, and Time (ZT). Data are presented as means ± SEM. Genes are considered rhythmic when p < 0.05 (Bold).