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. 2019 Jun 21;10:1406. doi: 10.3389/fimmu.2019.01406

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Copyright © 2019 Mándi, Endrész, Mosolygó, Burián, Lantos, Fülöp, Szatmári, Lőrinczi, Balog and Vécsei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the effect of kynurenic acid derivates on C. pneumoniae growth in U-937 cells based on quantitation of chlamydial DNA by qPCR. The cells were infected in 96-well plates at a MOI of 5 in a medium containing kinurenic acid derivates. Direct detection of C. pneumoniae ompA gene in the lysate of infected cells was performed at 24 (open bars) and 48 h (filled bars) postinfection. Fold change in the quantity of chlamydial DNA in kinurenic acid derivate treated cultures compared to the quantities detected in non-treated cultures was calculated. The mean of fold change in 3 parallel cultures and SD are shown. The differences are not significant.