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. 2019 Jun 20;10:1404. doi: 10.3389/fimmu.2019.01404

Figure 4.

Figure 4

The absorption of ingested LPS is greatly underestimated by limulus and fluorescent assays for LPS uptake. (A) Immunofluorescence of ileal villi 90 min after gavage with saline (Ctrl) or FITC-LPS (arrow, green). (B) Serum LPS 2.5 h after oral gavage with saline alone, Pam3CSK4 or E. coli LPS, as measured using the LAL assay (n = 4/gp). (C) Radiolabel in mouse tissues 4 h after oral gavage with 1 mg 3H-labeled LPS (n = 4/gp), measured in CPM (counts per minute). P-values vs. Ctrl (or blood levels of radiolabel for panel C), ANOVA with Dunnett's test.