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. 2019 Jun 28;10:733. doi: 10.3389/fphar.2019.00733

Figure 1.

Figure 1

C-phycocyanin dose-dependently reduced oxygen–glucose deprivation/reoxygenation (OGD/R)-induced cell death and reactive oxygen species (ROS) in H9c2 cells. (A) Experiment design. The group C0 was exposed to OGD/R and treated with sterile phosphate-buffered saline (PBS). Groups C10, C20, and C40 were treated with C-phycocyanin (C-pc) at the concentration of 10, 20, and 40 μg/ml, respectively, in addition to the exposure to OGD/R. Cells in the control groups were cultivated under the normal conditions without exposing to OGD/R. (B) Chemical structure of the C-pc (Bharathiraja et al., 2016). (C) Effects of C-pc on OGD/R-induced cell death. Cell viability was detected by cell counting kit-8 (cck-8) assay. (D) Effects of C-pc on OGD/R-induced intracellular ROS. (E) Effects of C-pc on mitochondrial function assessed by an ATP detection kit. The ATP level was normalized by protein levels (per milligram per milliliter). Data were expressed as mean ± SEM (n = 5). # P < 0.05, ## P < 0.01 versus control (normal) group; *P < 0.05, **P < 0.01 versus C0 (OGD/R model) group. P < 0.05 was considered as statistically significant.