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. 2019 Jun 28;10:733. doi: 10.3389/fphar.2019.00733

Figure 3.

Figure 3

C-phycocyanin reduced the expression of mitochondrial fission proteins DLP-1 and Fis1, suppressed DLP-1 phosphorylation, and transportation in H9c2 cells induced by OGD/R. (A) Representative Western blot images show the expression of mitochondrial fission proteins (FIS-1 and DLP-1) and DLP-1 phosphorylation. (B–D) Densitometric quantitation is shown as percentage protein expression of FIS-1 (B), DLP-1 (C), and P-DLP-1 (D). (E) Quantification analysis of the amount of DLP-1 in mitochondria (co-localization of DLP-1 and mitochondria). (F) Representative images from immunofluorescence staining of H9c2 cells treated with PBS or C-pc (40 μg/ml) to OGD/R exposure. The cells were immunostained with 4’,6-diamidino-2-phenylindole (blue), Mito-Tracker (red), and anti-DLP-1 antibody (green). Data were expressed as mean ± SEM (n = 5). # P < 0.05, ## P < 0.01 versus control (normal) group; *P < 0.05, **P < 0.01 versus C0 (OGD/R model) group. P < 0.05 was considered as statistically significant.