Table 1. Stability of the unique mutations in RNA extracted from semi-purified MuV–PP1 virus after 6 passages on Vero cells.
| Position nr in the genome | Change from G09 to PP1 | Effect in PP1 on protein, position and change | Number of reads in PP1 passage 6 | Variant reads | Number of reads in MuVG09 passage 6 | Variant reads |
|---|---|---|---|---|---|---|
| 2551 | C>U | P192 P>L; V191 P syn | 276 U | none | 1606 C | 1 A |
| 2611 | A>C | P212 Q>P V211 P syn | 207 C | 1 A; 2 U | 1171 A | 1 A; 1 C |
| 2654 | A>G | P226 R syn | 206 G | 3 A | 986 A | none |
| 2763 | C>U | P263 L syn | 222 U | none | 1544 C | 1 U |
| 2768 | U>A | P264 A syn | 232 A | none | 1312 U | 3 C; 1 G |
| 2780 | A>G | P268 G syn | 206 G | none | 1417 A | none |
| 2789 | G>A | P271 A syn | 265 A | 1 G | 1635 G | 1 U |
| 2810 | G>U | P278 P syn | 261 U | none | 2149 G | 2 U |
| 2816 | C>U | P280 N syn | 266 U | 1 A; 2 G | 1645 C | 2 A |
| 2864 | U>C | P296 H syn | 280 C | none | 1714 U | none |
| 2867 | A>G | P297 V syn | 294 G | none | 1609 A | none |
| Total | 2715 | 10 | 16788 | frequency 0.8 x 10−3 |
Syn = synonymous; in bold variants that would restore the original nucleotide in MuVG09