Table 2. Stability of the unique mutations in RNA extracted from semi-purified PP2 virus after 6 passages on B-LCL cells.

Position nr in the genome	Change from G09 to PP2	antigenome reads in PP2 passage 6	Variant reads	genome reads in PP2 passage 6	Variant reads
607	G > A syn	846 A	1 C	850 A	2 C
622	A > G syn	1031 G	none	1001 G	none
628	A > G syn	1067 G	4 U	1059 G	none
679	C > A syn	680 A	none	1506 A	1 G; 1 U; 1 C
697	U > G syn	692 G	none	2502 G	3 U
736	G > U syn	887 U	5 C; 2 G	2733 U	6 A; 1 C; 7 G
760	G > U syn	1093 U	4 C; 3 G	1594 U	1 G
784	A > G syn	975 G	3 A	1348 G	6 U; 2 A
841	U > C syn	1338 C	2 U; 2 A; 2 G	659 C	2 A
844	U > C syn	1400 C	3 A; 3 U	650 C	2 A; 1 G
850	U > C syn	1447 C	1 U; 1 G	632 C	1 G; 1 U
859	A > G syn	1444 G	2 U	523 G	none
860	C > U syn	1434 U	3 C; 3 G	489 U	none
Total frequency	cluster 1	14334	43	15546	37 1.04 x 10−3
1225	C > U syn	1325 U	2 C	620 U	1 C; 1 G
1240	G > A syn	1461 A	6 C; 1 U	514 A	1 C
1312	A > G syn	2819 G	2 U; 1 A	369 G	2 U
1327	C > U syn	2613 U	6 C; 2 G; 1 A	339 U	1 C
1330	U > C syn	2645 C	10 A; 8 U; 1 G	343 C	1 U
1354	A > G syn	2759 G	12 A; 2 C; 4 U	488 G	none
1366	G > A syn	2811 A	5 G; 2 C; 1 U	764 A	3 G; 1U
1369	A > G syn	2836 G	9 U; 3 A; 2 C	784 G	1 C; 1 U
1384	G > C syn	2596 C	6 A; 3 U	1195 C	3 A; 1 U
1387	G > A syn	2610 A	8 C; 2 U; 1 G	1322 A	1 U1]
1390	C > U syn	2584 U	4 A; 2 G	1429 U	3 A; 3 G
1413	G > A syn	3626 A	3 C; 2 U; 1 G	1612 A	3 U; 1 C
1438	G > A syn	3749 A	6 C; 5 G; 3 U	1553 A	8 C; 3 U; 1 G
1451	U > C syn	3508 C	5 A; 3 U; 2 G	1510 C	3 A; 1 U
1468	C > U syn	4457 U	5 C; 3 G; 2 A	982 U	4 C; 2 G; 1 A
1471	U > C syn	4496 C	3 A; 2 U	1167 C	2A; 1 U
1489	U > C syn	4259 C	7 U; 5 A	1352 C	2 U
1524	U > C N460 V>A	4359 C	5 A; 3 U	1265 C	4 A; 1 G; 1 U
1558	G > A syn	2910 A	3 U; 2 C; 1 G	729 A	1 G; 1 U; 1 C
Total frequency	cluster 2	58423	176	18337	65 1.05 x 10−3
Total	both clusters	72757	To original: 81 To other: 138 Other/original = 1.70	33883	To original: 31 To other: 71 other/original > 2.29

In bold variants that would restore the original nucleotide in MuV^G09