Table 3. Examples of novel mutations observed after 6 passages of PP1, PP2 and the parent MuVG09 virus.
| Virus/cell type | position nr | Passage 1 | Passage 6 | Effect on protein and position | Frequency |
|---|---|---|---|---|---|
| PP1/Vero | 849 | U | U>G | N235 F>C | 11.5% |
| 1178 | G | G>A | N345 V>I | 24.6% | |
| 3355 | A | A>G | M31 E>G | 52% | |
| 9014 | U | U>C | L193 I>T | 9.9% | |
| 12748 | C | C>U | L1437 syn | 20% | |
| PP2/B-LCL | 559 | U | U>C | N138 syn | 3% |
| 1896 | U | U>C | N3’UTR | 7% | |
| 1897 | U | U>A | N3’UTR | 6.6% | |
| 2710 | A | A>G | P245 Q>R | 33% | |
| 2859 | A | A>G | P295 D>N | 7.6% | |
| 8171 | G | G>A | HN238 G>S | 38% | |
| 8175 | U | U>G | HN239 L>R | 46% | |
| 2227 | U | U>C | P/V83 syn | 6% | |
| 2251 | U | U>C | P/V91 syn | 6% | |
| 2256 | U | U>C | P/V93 I>T | 6% | |
| 2260 | U | U>C | P/V94 syn | 6% | |
| G09/Vero | 1366 | G | G>U | N406 syn | 20.3% |
| 1481 | G | G>U | N446 D>Y | 18.5% | |
| 2710 | A | A>G | P245 Q>R | 20.8% | |
| 5638 | U | U>A | F365 Y>N | 38.7% | |
| 6704 | A | A>U | HN32 T>P | 17.4% | |
| 9896 | G | G>U | L487 D>Y | 45.4% |