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. 2019 Jul 5;14(7):e0219398. doi: 10.1371/journal.pone.0219398

Fig 2. NOXA expression in combination with ABT-263 enhances cell death in HN12 cells.

Fig 2

(A) Top: HN8 cells were treated with Ad-Con (C), Ad-NOXA (N) and/or ABT-263 (A) for 16 h and equal amounts of the total extracts were analyzed by Western blots with the indicated antibodies. Middle: Cells were treated with the same as above for 24 h and FACS analysis was performed to determine the amount of apoptosis (N = 3). Values represent the means ± S.D. Bottom: Cells were treated with the same as above for 72 h and stained with crystal violet. (B) HN12 cells were treated and analyzed as (A). Values represent the means ± S.D. for three independent experiments. (C) BAX and BAK are required for cell death induced by NOXA+ABT-263 in HN12 cells. Top: HN12 shBAK cells established in S1B Fig were infected with lentiviruses encoding shBAX or non-targeting control (shC) for 24 h. Cells were then treated with Ad-Con (C), Ad-NOXA (N) and/or ABT-263 (A) for 16 h and equal amounts of the total extracts were analyzed by Western blots with the indicated antibodies. Middle: Cells were treated with the same as above for 24 h and Annexin V-PI staining followed by FACS analysis was performed (N = 3). Values represent the means ± S.D.