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. 2019 Jul 5;14(7):e0219398. doi: 10.1371/journal.pone.0219398

Fig 5. NOXA or fenretinide in combination with ABT-263 increases cell death in HN30 cells.

Fig 5

(A) Top: HN30 cells were treated with fenretinide (10 μM) and/or ABT-263 (1 μM) for 16 h. Equal amounts of the total extracts were used for immunoblot analysis with the indicated antibodies. Middle: Cells were treated as above for 24 h and the amounts of apoptosis were determined by Annexin V-PI staining followed by FACS analyses (N = 4). Values represent the means ± S.D. Bottom: HN30 cells were treated for 72 h and stained with crystal violet. (B) HN30 cells were infected with lentivirus-encoding shRNA for non-targeting control (shC) or NOXA (shNOXA). Cells were then treated and analyzed as (A). Values represent the means ± S.D. for four independent experiments. (C) HN30 cells were infected with lentivirus-encoding shRNA for non-targeting control (shC) or p53 (shp53). Cells were then treated and analyzed as (A). Values represent the means ± S.D. for four independent experiments.