. 2019 Jul 5;14(7):e0219292. doi: 10.1371/journal.pone.0219292

Table 1. Details of probe and primers used in the RT-RPA assay.

Primer or probe	Sequence (5’-3’)	Position on genome^g
RPA_RV_N461F^a	`CAGGACAAAACACCGGCAACTATAAGACAAAC`	461
RPA_RV_N497F^b	`CAGATAGGATAGAGCAGATTTTCGAGACAGC`	497
RPA_RV_N528F	`CCCCTTTTGTTAAAATCGTGGAACACCATAC`	528
RPA_RV_N557F	`ACAAACATYGCRGATAGRATAGAGCAGATTTTY`	488
RPA_RV_N645R	`CTGATTGCTGAATATCTCTGCTCAATCCGG`	645
RPA_RV_N681R	`GAGCAGTCTTCATAAGCAGTGACAACTGTG`	681
RPA_RV_N692R	`GYTCAATCCGGGAGAAAWACATGTCRTTTCC`	622
RPA_RV_N562exoprobe	`ATGACAACTCAYAARATGTGYGCYAATTGGAGYAC(FAMdTc^c/THFd^d/BHQ-1dTe^e)ACCRAAYTT(C3Sp^f)`	562

^a R: reverse primer

^b F: forward primer

^c FAMdTC: thymidine nucleotide carrying 6-carboxyfluorescein

^d THFd: tetrahydrofuran residue

^e BHQ-1dTe: thymidine nucleotide carrying black hole quencher 1

^f C3Sp: C3 spacer to block elongation

^g Nucleotide positions are numbered according to rabies virus, CVS-11 (GenBank accession number GQ918139)