Fig 2. Detection of CsgD and CsgA protein synthesis in representative Salmonella strains by Western blot.

Whole-cell lysates were derived from multicellular aggregates [MA] or planktonic cells [PC] harvested from flask cultures of nontyphoidal and typhoidal Salmonella. Lysates used for CsgD detection (top panel) were normalized based on total protein concentration. Purified CsgD-6xHis recombinant protein was used as a technical control for CsgD detection. Pooled control samples were derived from combining lysates obtained from the multicellular aggregates of S. Typhimurium 14028 and SL1344 and S. Enteritidis 4931 and 301 strains. Black arrows indicate the detection of CsgA subunit dimers (D) and monomers (M) (bottom panel). The data shown is representative of two biological replicates.