Fig 2. Ligand repertoires determined for three Siglec-like BRs.

The high affinity sialoglycan ligands for the recombinant BRs were determined by analysis on a custom sialoglycan array and by enzyme-linked assays [23, 26]. The O-glycan ligand preferences were determined by analyzing glycan profiles of affinity-captured plasma proteins [33]. Note that 10712_BR was previously referred to as NCTC10712_BR. Binding of strains M99 and DL1 to array glycans paralleled binding of the respective recombinant BRs [26]. Monosaccharide symbols follow the Symbol Nomenclature for Glycans system [67]. Neu5Ac, N-acetyl neuraminic acid; Gal, galactose; GalNAc, N-acetyl galactosamine; GlcNAc, N-acetyl glucosamine; Fuc, fucose. sTa, sialyl T-antigen; sLe^C, sialyl Lewis C; 3'sLn, 3'sialyllactosamine; sLe^X, sialyl Lewis X. Throughout the manuscript, 3'sLn is referred to simply as "sLn". The "core 1" and "core 2" designations refer to the protein-proximal glycan structures.