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. 2019 Jun 24;15(6):e1007896. doi: 10.1371/journal.ppat.1007896

Fig 3. Design of isogenic variant strains of S. gordonii M99.

Fig 3

Strains were designed to express GspB variants in which amino acid residues 248 to 604 of GspB (GenBank accession number AAL13053) were replaced with residues 242 to 448 of Hsa (ABV10391) or 241 to 446 of the SRR glycoprotein homolog from S. gordonii UB10712 (WP_045635027). A: Alignment of the BR domain junctions. Conserved amino acids are indicated in red type. T248 and S604 of GspB, A242 and Q448 of Hsa, and V241 and Q446 of the UB10712 homolog are underlined. B: Strategy to replace the GspB BR coding region in the native S. gordonii chromosomal locus. PS2114 is a derivative of S. gordonii M99 that has a deletion of gspB codons 1 to 486 and a cat gene in the upstream non-coding region [30]. Chimeric sequences were introduced into the S. gordonii chromosome via a strategy that involved recombination by double-crossover between gspB codons 605–703 and the upstream pdxU gene.