Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 1;12:1833–1852. doi: 10.2147/IDR.S210890

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Jasso-Miranda et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Effect of quercetin and fisetin on macrophages infected with DENV-3. Macrophages were infected with DENV-3 at a MOI of 1 (A–C) or with mixtures of DENV-3 and 4G2 antibody (ADE) at 1:256 dilution (D–F). Viral inoculum was discarded after 1 hr of infection and fresh medium was added with 100 µM of quercetin or 40 µM of fisetin. Untreated infected macrophages were included for comparison (A and D). Cell supernatants were collected at different times postinfection and the concentrations of IL-6, TNF-α, IL-10 and IFN-γ were determined by ELISA. Data represent average ± SD of two independent and duplicated experiments; nonlinear regression analysis was performed using GraphPad Prism 5.0 to obtain trend lines.

Abbreviations: DENV, dengue virus; MOI, multiplicity of infection.