Fig. 3.
a Binding of various concentrations of CRP in diluted human saliva to 0.1 mg/ml anti-CRP immobilized onto Au slide along with the calibration curves for various concentrations of CRP. b CRP detection in a diluted sample of human saliva. The CRP/anti-CRP dose curve (red squares) measured in diluted human saliva is shown as the relative change of the ID current (ΔI/I0) vs. the saliva dilution factor. A functionalized gate comprising both the anti-CRP and the BSA is used. The black circles are the negative control responses of a bare BSA-functionalized gate to CRP in diluted human saliva. The full red line is the result of the SiMoT modeling. The data points are relevant to three replicates of the dose curves, while the error bars have been evaluated as the relative standard deviation