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. 2019 Jul 5;9:9762. doi: 10.1038/s41598-019-46114-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ang II and Ang-(1–7) synthesis in the transplanted kidney. Contribution of ACE (A) and chymase (B) activity to form Ang II from Ang I was measured in kidney biopsy homogenates of KTx patients with various graft vintage. After spiking the samples with Ang I as substrate, NEP enzyme activity to form Ang-(1–7) in kidney biopsy homogenates of KTx patient groups by various graft vintage without RAS blockade (no RASi) or with ACEi therapy was analysed by mass spectrometry. (C) Similarly, PEP activity was determined (D).