Fig. 3.

Candidate genes directly affect TNBC cell migratory behavior. a Quantification of single cell migration speed of Hs578T-GFP cells after knockdown of validated hits. Hs578T-GFP cells were transfected with siRNAs and cell migration was assessed by live microscopy. Hits that showed significant and consistent effects in both replicates were considered as candidate genes (right panel). Median ± 95% confidence interval is shown and cell populations were compared by Kruskal–Wallis test with Dunn’s post correction test. b Same as in a, with MDA-MB-231-GFP cells. c Single cell trajectories of cell migration upon knockdown of PRPF4B, MXD1, BUD31, or BPTF in (i) Hs578T and (ii) MDA-MB-231 cell lines